Chinese herbal medicine Dahuapao is the root or whole plant of the Rosaceae plant Dahuapao. The medicinal material can be harvested throughout the year and can be used after drying. It has the effects of clearing heat, stopping bleeding, and dispelling wind and dampness. Now let's study the analgesic effect of water extract of Dahuapao.
Dahuapao image
Purpose: To study the analgesic effect of water extract of Dahuapao on mice and its effect on NO content in serum.
Methods: Fifty mice were selected for the hot plate-induced pain method and the acetic acid writhing method, respectively. Each group of mice under each method was divided into five groups, namely, the high-dose group of Dahuapao root water extract (5.2 g/kg crude drug), the medium-dose group (2.6 g/kg crude drug), the low-dose group (1.3 g/kg crude drug), the positive control group (2.0 mg/kg morphine hydrochloride), and the negative control group (physiological saline).
The hot plate-induced pain method observed the changes in the thermal pain threshold and serum nitric oxide (NO) content in mice, and the acetic acid writhing method observed the latent period of writhing, the number of writhing episodes, the analgesic inhibition rate, and serum NO level in mice.
Results: Compared with the negative control group, the thermal plate method showed that the pain threshold of mice in the positive control group increased, serum NO level decreased. The acetic acid writhing method showed that the latent period of writhing in mice increased, the number of writhing episodes decreased, and the analgesic inhibition rate increased in the positive control group, with statistically significant differences (P<0.05), indicating that the mouse models in the hot plate-induced pain method and the acetic acid writhing method were successful.
In the hot plate method, compared with the negative control group, the high, medium, and low dose groups of Dahuapao water extract increased the pain threshold of mice and decreased serum NO content, with statistically significant differences (P<0.05). In the acetic acid writhing method, compared with the negative control group, the high and medium dose groups of Dahuapao root water extract increased the latent period of writhing in mice, decreased the number of writhing episodes and increased the analgesic inhibition rate within 10 minutes, and the high and medium dose groups of mice had decreased serum NO content, with statistically significant differences (P<0.05).
Conclusion: The water extract of Dahuapao has analgesic effects, and its mechanism of action may be related to the reduction of NO content in mouse serum.
